Summary
Type: SPE
Application #: 300410
Catalog#: 730073 - SPE Cartridges, Chromabond, Silica, 3ml, PP, 500mg, 50/pk
Detection: For further analyses we recommend HPLC using column NUCLEOSIL® 100-5 C8 in reference to Appl. No: 106910
Conditions: Column conditioning:
Place 2 g dry sodium sulphate onto the SiOH column (a combination column SiOH / Na2SO4 is available on request). Force or aspirate 5 ml n-hexane through the column, followed by 5 ml dichloromethane
Sample application:
Slowly force or aspirate the pretreated sample through the column
Column washing:
10 ml dichloromethane, then 10 ml n-hexane, finally 10 ml toluene
Elution:
8 ml toluene / acetone (95 : 5, v/v): zearalenone
6 ml toluene / glacial acetic acid (90 : 10, v/v): ochratoxin A
Eluates can be concentrated in a stream of nitrogen
Authors: Macherey-Nagel
Source: LC department